Part:BBa_K3245003:Design

Using homologous recombination to knockout the hns sequence from Nissle1917

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 482
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 648
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1312
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1847
  Illegal SapI.rc site found at 1161
  Illegal SapI.rc site found at 1371
  

Design Notes

Homologous arm need to be as long as possible

Source

Nissle1917 genome